CoaChrom^®
α1-Antitrypsin

Description

This kit is designed for the assay of α1-antitrypsin (α1-AT, α1-proteinase inhibitor) in human plasma. Plasma is diluted in buffer containing methylamine, which inactivates α2-macroglobulin. Excess trypsin is added and during an incubation period, it complexes with α1-AT. The remaining free trypsin is measured by its ability to cleave a chromogenic peptide substrate. The release of p-nitroaniline (pNA) from the substrate is measured at 405nm, and the log absorbance is inversely proportional to the plasma α1-AT concentration.

Intended use

Assay proposed for the measurement of α1-antitrypsin (α1-AT, α1-proteinase inhibitor) in human plasma. For research use only.